24-Hour Urine Cortisol Calculator

Introduction: The 24-Hour Urine Cortisol Calculator is a tool designed to estimate the concentration of cortisol in a 24-hour urine collection. It requires inputs such as urine volume and the amount of cortisol.

Formula: The formula for calculating cortisol concentration is: Cortisol Concentration=Amount of CortisolUrine VolumeCortisol Concentration=Urine VolumeAmount of Cortisol​

How to Use:

  1. Enter the urine volume (in mL) and the amount of cortisol (in mcg).
  2. Click the “Calculate” button.
  3. The result will be displayed below the button.

Example: If you input 2000 mL urine volume and 50 mcg of cortisol, the calculator will display “Cortisol Concentration: 0.0250 mcg/mL.”


  1. Q: Why measure cortisol in urine? A: Urine cortisol levels help assess adrenal gland function and diagnose conditions like Cushing’s syndrome.
  2. Q: What is the normal cortisol concentration in urine? A: Normal values vary, but typical concentrations are in the range of 20 to 90 mcg/24 hours.
  3. Q: Can this calculator diagnose adrenal disorders? A: It provides an estimate, but a healthcare professional’s evaluation is necessary for a comprehensive diagnosis.
  4. Q: How is a 24-hour urine collection done? A: Follow your healthcare provider’s instructions for accurate results. It involves collecting all urine over a 24-hour period.
  5. Q: Are there factors that can affect cortisol levels? A: Yes, factors such as medications, stress, and certain medical conditions can influence cortisol levels.

Conclusion: The 24-Hour Urine Cortisol Calculator aids in estimating cortisol concentration, offering valuable information for the assessment of adrenal function. Any unusual results should be discussed with a healthcare professional for further evaluation and diagnosis.

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